More

EDF在线老虎机 project, providing synthesis, ADME-Tox profiling, medicinal chemistry and logistic services.

The Coronavirus Library consists of 7 sublibraries which can be also acquired separately.

Most popular library formats

Catalog No.
Target
Compounds
Amount
Format*
Price

Catalog No.

COV-16800-0-Z-10

Target

3CLpro, ACE2, TRPMSS11E and all below

Compounds

16 800
14 plates

Amount

up to 300 nL of 10 mM DMSO stock solutions

Format

1536-well plates, 1280 compounds per plate, 1-4 and 44-48 columns empty

Request a quote

Request a quote

Catalog No。

MPR-3440-10-Y-10

EDF在线老虎机Catalog No。

Main protease Mpro, non-covalent compounds

Compounds

3 440
11 plates

Amount

10 µL of 10 mM DMSO stock solutions

Format

384-well plates Greiner Cat. No. 781280, 320 compounds per plate, 1, 2 and 23, 24 columns empty

Request a quote

Request a quote

Catalog No.

MPC-2640-50-X-20

Catalog No.

Main protease Mpro, covalent compounds

Compounds

2 640
33 plates

Amount

50 µL of 20 mM DMSO stock solutions

Format

96-well plates, Greiner Cat. No. 650201, 80 compounds per plate, 1 and 12 columns empty

Request a quote

Request a quote

Catalog No。

TMP-3200-25-Y-10

Catalog No.

EDF在线老虎机Type-II and 11e transmembrane serine protease (TMPRSS2)

Compounds

3 200
10 plates

Amount

25 µL of 10 mM DMSO stock solutions

Format

384-well plates Greiner Cat. No. 781280, 320 compounds per plate, 1, 2 and 23, 24 columns empty

Request a quote

Request a quote

Catalog No.

PLP-3200-50-Y-10

Catalog No.

Papain-like protease (PLpro)

Compounds

3 200
10 platesEDF在线老虎机

Amount

50 µL of 10 mM DMSO stock solutions

Format

384-well plates Greiner Cat. No. 781270, 320 compounds per plate, 1, 2 and 23, 24 columns empty

Request a quote

Request a quote

Catalog No。

RDR-2880-50-X-10

Catalog No.

RNA-dependent RNA polymerase (RdRP)

Compounds

2 880
36 plates

Amount

50 µL of 10 mM DMSO stock solutions

Format

96-well plates, Grainer Cat. No. 650201, 80 compounds per plate, 1 and 12 columns empty

Request a quote

Request a quote

*Please request for any other options through our contact form. We will be happy to deliver our library in any convenient for your project formats.

Library Design

To design our library we carefully collected all available structural information of most promising SARS-CoV protein targets. The protein structures were scrutinized and docking models were created. The docking models were validated by short MD simulations and verified then by ability to form complexes with reported active molecules. We have focused on the following 5 targets and NSP, reported to be most important for virus transmission and essential for viral replication:

  • SARS-CoV-2 main protease Mpro (also called 3CLpro)
  • RNA-dependent RNA polymerase (RdRp)
  • Papain-like protease (PLpro)
  • Angiotensin-converting enzyme 2 (ACE2) receptor
  • Type-II transmembrane serine protease (TMPRSS2)
  • Non-structual proteins (NSP) of SARS-CoV-2, with reported 3D-struture.

For cysteine and serine proteases (Mpro and TMPRSS2) covalent docking has been carried out to identify promising covalent binders, which can elongate inhibition action. The databases of screening compounds were preliminary filtered to contain only compounds with specific warheads that are selective to each amino acid:

Mpro focused Covalent sublibrary
TMPRSS2 focused Covalent sublibrary

sublibrary1

2 640 compounds (acrylamides, chloroacetamides, vinyl sulfones and beta-lactams)

sublibrary2

560 compounds (sulfonyl fluorides, N-nitriles, chloroacetamides, epoxides, boronics)

All other target-focused sublibraries passed stringent MedChem filters, including PAINS and Molecular Parameters restrictions to represent mainly lead-like space. The hits derived from our library can be easily followed with analogs and growing strategy to maximize MedChem variability within the structure.

Molecular Profile of the Library

Examples of the docking results

Binding poses of two predicted hit molecules after docking calculation on Mpro protease Z1609752806 (light blue ligand on the left side) and Z1143050660 (grey ligand on the right side).

SARS-CoV-2 NSP16 protein bound to NSP10. All 3 binding pockets were used for molecular docking calculations.

The example of NSP16 SAM binding pocket docking result. NSP16 in grey, docked ligand in green, SAM molecule in yellow.

FOLLOW US

皇冠足彩APP 天天斗牛 体育赛事 山东群英会app下载 新皇冠体育网